Figure 4: ARMS plate image analysis step-by-step process of extracting Didemnum percent coverage data. A: Original image, scale of image programed into ImageJ. B: Contrast increased and brightness decreased to aid software in distinguishing white Didemnum from pale grey plate background. C: Trainable segmentation plugin initiated to recognize Didemnum. D: Black and white composite image generated by segmentation software. Program edited if necessary to eliminate larger areas incorrectly identified as Didemnum E: Particles analyzed and converted to % Area output, parameters set to particles greater than 15mm² to eliminate noise and inconsistencies. Source: Author